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Pathogenic Microbiology and Immunology for Health Professions, BIO 261 TEXTBOOKS: Textbook: Selected Chapters From Microbiology A Human Perspective By Eugene W. Nester, Denise G. Anderson, C. Evans Roberts Jr. and Martha T. Nester, Medgar Evers College Custom ed. LAB MANUAL Selected Chapters From Benson’s Microbiological Applications Laboratory Manualin General Microbiology by Alfred Brown, and Laboratory Manual and Workbook in Microbiology Application to Patient Care by Josephine A. Morello, Helen E. Mizer, and Paul A. Granato, Medgar Evers College Custom ed. COURSE DESCRIPTION: A course which emphasizes the basic medical microbiology principles and infectious diseases. The first part of the course will detail the fundamental principles of microbiology, including microbial structure and function, microbial ecology and microbiological techniques. The second part will include microbe-host interaction, mechanism of pathogenicity, immunology, antisepsis and antimicrobial drugs. The last part will cover the common infectious diseases of human. COURSE REQUIREMENTS: Students must have finished the general biology course requirement e.g. BIO.111, BIO. 201 and BIO.202 or BIO 251 and 252. Attendance to lecture and laboratory classes is required. Any student who is absent from a class meeting is responsible for all class work and announcements made by the professor including test dates. Students should make every effort to be in class on time, listen attentively, take notes, study the textbook and ask questions when necessary. Students are also encouraged to form study groups and help each other. Tutorial services are available. Students are urged to to seek the help of their professor in the event that they have problem that need attention. In addition to the office hour, your professor may, on request ,be available for conferences before or after class. There will be quizzes and at least three hourly test given by individual instructors during the semester. There will also be a uniform course midterm exam for all sections and a uniform course final exam at the end of the semester. THERE WILL BE NO MAKE-UP EXAMS FOR THE COURSE MIDTERM OR COURSE FINAL EXAMS GRADING: The lecture component of the course will account for 60% of the final grade distributed as follows: Class quizzes and hourly exams (25%), course midterm exam (15%), course final exam (20%). The lab component of the course will account for 40% of the final grade. LECTURE TOPICS Introduction: Definition, Evolution, History & Characteristics of microorganisms. Basic structures of microorganisms, different types of microbes. The Eucaryotic Microorganisms, :Protozoa, Fungi & Animal Parasites. Microbial life, microbial activities, culture, kinetics of microbial growth; growth requirements & environmental activities of microbes. Tools, techniques & methods used in the study of microorganisms, aseptic techniques, sterilization; microscopy, staining; biochemical methods including serology. Epidemiology & Microbial Diseases: Host-parasite relationships, infection & pathogenicity and virulence. Host Defense Mechanisms: Non specific defense mechanism, specific resistance/Immunity to infection, antigens-antibodies reaction, overall review of the immune system. Disorder and Limitations of the Immune System: auto-immune diseases, allergic reaction, hypersensitivity. Source & Diagnosis of Infectious Diseases. Prevention and Control of Infectious Diseases. Antimicrobial Agents: antiseptics & antibiotics. Treatment of Infections: antibiotics and antiviral drugs. Bacterial diseases acquired through the respiratory tract, upper respiratory tract infections, lower respiratory tract infections, Viral diseases acquired through the respiratory tract. Bacterial diseases acquired through the alimentary route. Viral diseases acquired through the alimentary route. Parasitic diseases of the digestive canal caused by protozoa & helminths. Infectious diseases acquired through the skin and mucosa and transmitted by human contact (direct skin contact, sexually transmitted diseases), by animals or from the environment and by faulty medical & surgical procedure Diseases acquired through vectors. Control of communicable diseases in the community & hospitals. Laboratory Topics The microscope Handling and examining cultures Simple stains: Gram, Acid Fast, Special stains Pure culture techniques Culturing microorganisms from the environment Primary media for isolating microorganisms, how to prepare media Simple carbohydrate fermentation, starch hydrolysis, production of indole, hydrogen sulfide, motility Activity of urease, catalase, gelatinase, deoxyribonuclease, deaminase Disinfectants Agar Disk Diffusion Protozoans and animal parasites, yeast and other fungi Streptococci, Pneumococci, Staphylococci, Enteric pathogens, culturing a fecal sample Quantitative urine cultures Enterotubes Neisseria, spirochetes, anaerobes, mycoplasma, rickettiae, chlamydia and viruses Serologic identification of microorganisms Serologic identification of patients' antibodies
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